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Bioimaging encompasses the techniques and tools employed to capture, analyze, and display structural or functional images of biological specimens or systems across various spatial and temporal resolutions. This field also includes methods for examining preserved biological samples. Critical components of bioimaging involve image processing, data mining, and large-scale data analysis. A fundamental principle of bioimaging is minimizing disruption to natural biological processes, typically employing non-invasive approaches that frequently deliver real-time insights. The scope of visualization spans from microscopic cellular components to complete organisms, encompassing tissues and organs. Bioimaging relies on electromagnetic and ultrasonic energy sources for image generation. Widely used imaging techniques include x-rays, computed tomography (CT), positron emission tomography (PET), magnetic resonance imaging (MRI), ultrasound, photoacoustic imaging, optical coherence tomography, along with fluorescence and optical microscopy. Additionally, specialized substances like contrast agents, therapeutic compounds, molecular probes, and nanosensors play vital roles in bioimaging, enabling the study of molecular and cellular mechanisms while facilitating the creation of advanced diagnostic methods and innovative treatments.