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Our research focuses on investigating the biological characteristics of human mesenchymal stem cells (MSCs) in both healthy and diseased states, which is essential for creating innovative stem cell therapies. We study the in vivo characteristics and distribution patterns of MSCs and their potential applications in osteoarthritis treatment. Our work examines the use of minimally processed MSCs for immunomodulatory purposes, particularly in bone regeneration and cartilage repair. Enhancing osteoarthritis care involves earlier detection, better non-surgical treatments, tissue-preserving approaches like acellular scaffolds, more durable joint replacements, and personalized treatment strategies that ensure greater precision in medical device design and treatment delivery, along with improved treatment consistency.